Understanding Separation Techniques

This is an MCQ-based quiz for GRE on the topic of Separation Techniques.

This includes Distillation, Crystallization, Adsorption, Membrane procedures, Absorption and Stripping, and Oxidation.

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A student co-immunoprecipitates the NF-kappa B transcription factor that is bound to a protein, which the student cannot identify. What technique would be suitable for identifying this unknown protein?

Immunohistochemistry Western blot Mass spectrophotometry Electroporation Electrophoretic mobility shift assay (ESMA)

Which of the following stains could be used to identify bacteria?

Acid fast stain Giemsa stain Gram stain Eosin stain Wright stain

Which of the following is a method for sample introduction into a capillary electrophoresis system?

Electrokinetic injection Siphoning Hydrodynamic injection All of these Pressure injection

Compared to slab gel electrophoresis, which is an advantage of capillary electrophoresis?

Reproducibility of matrix viscosity Data collection can be done in real-time Automatable sample loading Less sample consumption All of these

Capillary electrophoresis instrumentation involves which of the following components?

Polymer Positive and negative electrodes Detector Buffer system All of these

What is the purpose of a electrophoretic mobility supershift assay?

To determine if a certain protein binds a DNA sequence To determine if two proteins interact with one another To separate DNA fragments by size To separate proteins by size To separate proteins by charge

Which technique is best suited to determine if a protein is active and able to bind DNA?

Electrophoretic mobility shift assay (EMSA) Northern blot Western blot Bradford assay Southern blot

In polymerase chain reaction (PCR), the reaction mixture is heated to approximately 98°C during the first cycling step in the procedure. Which of the following describes the purpose of this step?

Degradation of non-target sequences within the template Activation of Taq polymerase Bringing the reaction to the optimal temperature for annealing of the primers to the template Solubilizing the dNTPs Melting of the DNA template, producing single-stranded DNA to which primers can bind

When would it be appropriate to use extraction in order to separate compounds in a solution?

When the compounds have differing conjugated double bond lengths, but similar molecular weights. When the compounds have differing molecular weights, but similar solubilities. When the compounds have similar polarities, but differing solubilities. When the compounds have similar molecular weights, but differing polarities.

A student researcher wants to determine if two proteins physically interact within a cell. What technique is best suitable for this experiment?

Co-immunoprecipitation Immunoprecipitation Cross-linking Chromatin interaction analysis by paired-end tag sequencing Chromatin immunoprecipitation sequencing

Quiz/Test Summary

Title: Understanding Separation Techniques

Questions: 10

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