Understanding Separation Techniques

Immunohistochemistry Western blot Mass spectrophotometry Electroporation Electrophoretic mobility shift assay (ESMA) Acid fast stain Giemsa stain Gram stain Eosin stain Wright stain Electrokinetic injection Siphoning Hydrodynamic injection All of these Pressure injection Reproducibility of matrix viscosity Data collection can be done in real-time Automatable sample loading Less sample consumption All of these Polymer Positive and negative electrodes Detector Buffer system All of these To determine if a certain protein binds a DNA sequence To determine if two proteins interact with one another To separate DNA fragments by size To separate proteins by size To separate proteins by charge Electrophoretic mobility shift assay (EMSA) Northern blot Western blot Bradford assay Southern blot Degradation of non-target sequences within the template Activation of Taq polymerase Bringing the reaction to the optimal temperature for annealing of the primers to the template Solubilizing the dNTPs Melting of the DNA template, producing single-stranded DNA to which primers can bind When the compounds have differing conjugated double bond lengths, but similar molecular weights. When the compounds have differing molecular weights, but similar solubilities. When the compounds have similar polarities, but differing solubilities. When the compounds have similar molecular weights, but differing polarities. Co-immunoprecipitation Immunoprecipitation Cross-linking Chromatin interaction analysis by paired-end tag sequencing Chromatin immunoprecipitation sequencing