This is an MCQ-based quiz for GRE on the topic of Electrophoresis And Blots.
An electrophoretic system consists of two electrodes of opposite charge (anode, cathode), connected by a conducting medium called an electrolyte. There are three types of blotting methods: Southern blotting, Northern blotting, and Western blotting.
When performing a western blot, what is the purpose of adding a secondary antibody?
A student researcher overexpresses an exogenous protein in cell culture and wants to determine if that protein, is in fact, overexpressed. What technique would best demonstrate that this protein is expressed in these cells?
After proteins are run on an SDS-PAGE gel, a transfer is executed. What is the purpose of the transfer in Western blot protocol?
Which of the following is not a similarity between enzyme-linked immunosorbent assays (ELISAs) and western blots, two common protein detection methods?
A researcher is working with a protein that contains four subunits of differing molecular weights. If the researcher performs SDS-PAGE, how many distinct bands should he see on the gel?
Which of the following is a primary factor that dictates how far a protein will migrate during SDS-PAGE?
Which of the following is true about SDS-PAGE?
As opposed to electrophoresis with a more standard agar gel, what does polyacrylamide gel electrophoresis (PAGE) allow for when working with DNA?
Which of the following techniques would be most useful to study gene expression?
Which of the following probes are most commonly used in southern blotting?