Lab Techniques: DNA Analysis

This is an MCQ-based quiz for GRE on the topic of DNA Analysis.

The DNA testing process is comprised of four main steps, including extraction, quantitation, amplification, and capillary electrophoresis.

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When performing recombinant DNA techniques, it is often necessary to cut desired DNA sequences with restriction enzymes. Where are these enzymes typically isolated from?

They are synthetic Bacteria Fungi Plants Animals

Which of the following DNA sequences could most likely be cleaved by an endonuclease?

5'-TATACC-3'3'-ATATGG-5' 5'-GGAACC-3'3'-CCTTGG-5' 5'-CGATTA-3'3'-GCTAAT-5' 5'-CCTAGG-3'3'-GGATCC-5'

After a double digest with EcoRI and HindIII, a gel electrophoresis shows that you have several restriction fragment bands. You see bands of lengths 3kb, 4kb and 5kb. From previous tests, you know that there are two EcoRI restriction sites and two HindIII restriction sites in the DNA fragment you are studying. How many restriction fragments are really in your reaction?

Six It cannot be determined from the given information Four Three Five

Which of the following accounts for the inability of dideoxynucleotide triphosphates to further polymerize in Sanger DNA sequencing?

Lack of a triphosphate moiety on the 5' carbon Lack of a hydroxyl group on the 3' carbon Presence of a hydroxyl group on the 2' carbon Lack of a hydroxyl group on the 2' carbon Presence of a hydroxyl group on the 3' carbon

A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe?

The reaction will work, but the product will contain many undesired mutations The reaction will be completely unsuccessful The reaction will work, but amplify a region that was not his target The reaction will work, but at a significantly slower rate

Traditional Sanger style DNA sequencing relies on a method called chain termination. What type of molecule is used to terminate DNA chains to create molecules of all possible lengths covering the fragment to be sequenced?

Cyclic nucleotides Purines Dideoxynucleotides Deoxyadenosine Deoxyribonucleotides

Which of the following is a reason that cDNA clones of eukaryotic genes are capable of being expressed in prokaryotic cells? 
I. cDNA clones do not contain any of the introns present in the genomic DNA
II. Prokaryotes have the basic translational machinery needed to express these genes
III. Prokaryotes are capable of making similar post-transcriptional modifications as eukaryotes

I, II, and III

I only

I and II

II and III

rtPCR quantification method is a technique that __________.

Determines the melting point of the DNA fragments during each cycle of PCR

Separates DNA fragments based on their size

Determines if the primers used in the PCR reaction are annealing

Measures the amount of non-specific amplification products

Measures the amount of fluorescence produced which is proportional to DNA concentration with each cycle of PCR

The determination of the order of the four bases—adenine, thymine, cytosine, and guanine—in a strand of DNA is termed __________.

DNA sequencing

Gene flow

DNA amplification

Genetic recombination

DNA replication

A student researcher wants to change five consecutive base pairs in the middle of a PCR amplified fragment of DNA. What technique is best suited for this experiment?

DNA ligation None of the other answers Electrophoretic mobility shift assay (ESMA) Restriction enzyme digestion PCR mutagenesis
Quiz/Test Summary
Title: Lab Techniques: DNA Analysis
Questions: 10
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