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This is an MCQ-based quiz for GRE on the topic of DNA Analysis.
The DNA testing process is comprised of four main steps, including extraction, quantitation, amplification, and capillary electrophoresis.
When performing recombinant DNA techniques, it is often necessary to cut desired DNA sequences with restriction enzymes. Where are these enzymes typically isolated from?
Which of the following DNA sequences could most likely be cleaved by an endonuclease?
After a double digest with EcoRI and HindIII, a gel electrophoresis shows that you have several restriction fragment bands. You see bands of lengths 3kb, 4kb and 5kb. From previous tests, you know that there are two EcoRI restriction sites and two HindIII restriction sites in the DNA fragment you are studying. How many restriction fragments are really in your reaction?
Which of the following accounts for the inability of dideoxynucleotide triphosphates to further polymerize in Sanger DNA sequencing?
A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe?
Traditional Sanger style DNA sequencing relies on a method called chain termination. What type of molecule is used to terminate DNA chains to create molecules of all possible lengths covering the fragment to be sequenced?
Which of the following is a reason that cDNA clones of eukaryotic genes are capable of being expressed in prokaryotic cells?
I. cDNA clones do not contain any of the introns present in the genomic DNA
II. Prokaryotes have the basic translational machinery needed to express these genes
III. Prokaryotes are capable of making similar post-transcriptional modifications as eukaryotes
I, II, and III
I only
I and II
II and III
rtPCR quantification method is a technique that __________.
Determines the melting point of the DNA fragments during each cycle of PCR
Separates DNA fragments based on their size
Determines if the primers used in the PCR reaction are annealing
Measures the amount of non-specific amplification products
Measures the amount of fluorescence produced which is proportional to DNA concentration with each cycle of PCR
The determination of the order of the four bases—adenine, thymine, cytosine, and guanine—in a strand of DNA is termed __________.
DNA sequencing
Gene flow
DNA amplification
Genetic recombination
DNA replication
A student researcher wants to change five consecutive base pairs in the middle of a PCR amplified fragment of DNA. What technique is best suited for this experiment?
