This quiz contains multiple-choice problems on RNA structures, RNA genomes, RNA structure modelling applications, RNA secondary structure prediction methods, ab initio and comparative approach, performance evaluation, prediction limitations, energy method and free grammars.
RNA structures can be experimentally determined using
X-ray crystallography techniques only
NMR techniques only
X-ray crystallography or NMR techniques
Gel electrophoresis
Which of the following is not a form of RNA?
mRNA
tRNA
qRNA
rRNA
Unlike DNA, which is mainly double-stranded, RNA is double-stranded, although an RNA molecule can self-hybridize at certain regions to form partial double-stranded structures. True or false?
True
False
Structures are not very important when it comes to the study of functions. True or false?
True
False
Which of the following is not an RNA base?
Thymine (T)
Adenine (A)
Cytosine (C)
Guanine (G)
Base pairing in RNA is A–G and U–C. True or false?
True
False
In addition to the canonical base pairing, non-canonical base pairings often exist, such as __ and __ base paring.
G, U
G, C
U, C
A, C
Four main subtypes of secondary structures can be identified. They are hairpin loops, bulge loops, interior loops, and multi-branch loops. True or false?
True
False
The __ refers to a structure with two ends of a single-stranded region (loop) connecting a base-paired region (stem).
Helical junctions
Hairpin loop
Bulge loop
Interior loop
The __ refers to two single-stranded regions connecting two adjacent base-paired segments on opposite strands.
Hairpin loop
Interior loop
Pseudoknot loop
Helical junctions
Which of the following is true about the RNAalifold?
Dynamic programming is not involved
Minimum free energy method is not used
Only minimum free energy is used
Covariation information is taken into consideration
The predefined alignment requiring method also depends on the quality of the input alignment. If there __ errors in the alignment, covariation signals __ detected.
are, will be
are, will not be
are not, will not be
are not, possibly will not be
The type of algorithm that __ predefined alignment is __ for reasonably conserved sequences.
does not require, more successful
requires, less successful
does not require, relatively successful
requires, relatively successful
The type of algorithm that requires predefined alignment requires the user to provide __ alignment as input.
Not necessarily an alignment
Multiple only
Pairwise or multiple
Pair wise only
An aspect of the comparative method is to select a __ structure through consensus drawing.
Relatively distinct
Remote
Common
Least abundant