Functional Genomics and Proteomics

This approach is much more efficient than the EST analysis

This approach is much less efficient than the EST analysis

It uses a short nucleotide tag to define a gene transcript

It allows sequencing of multiple tags in a single clone

One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification

Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all

Correctly sequenced SAGE tag always corresponds to several genes

The drawback with this approach is the sensitivity to sequencing errors

Collecting a SAGE library is very labor intensive and expensive

Collecting a SAGE library is quite economical

SAGE is not suitable for rapid screening of cells

Gene identification from SAGE data is also more cumbersome

True

False

True

False

SAGE and DNA microarrays are both high throughput techniques that determine global mRNA expression levels

Studies indicate that the gene expression measurements from these methods are highly inconsistent with each other

SAGE does not require prior knowledge of the transcript sequence

DNA microarray experiments can only detect the genes spotted on the microarray

It is an NCBI web-based program

It allows matching of experimentally obtained SAGE tags to known genes

It provides an interface for visualizing human gene expression

It does not filter out linker sequences from experimentally obtained SAGE tags

True

False

True

False

Sequencing is the most costly and time-consuming step

Here, sequencing is economical but time-consuming step

Sequencing is economical but time-reducing step

It is difficult to know how many tags need to be sequenced to get a good coverage of the entire transcriptome

It stands for two-dimensional polyacrylamide gel electrophoresis

It separates proteins by charge only

The gel is run in one direction in a pH gradient under a non-denaturing condition

It works to separate proteins by isoelectric points (pI)

True

False

True

False

The proteolysis does not generate a pattern according to molecular weight

Proteins can be identified and characterized using MS

The proteins from a two dimensional gel system are first digested in situ with a protease

Protein spots of interest are excised from the two-dimensional gel

MS characterization of proteins is highly dependent on bioinformatic analysis

Bioinformatics programs can be used to search for the identity of a protein in a database of theoretically digested proteins

Even in reality, the protease digestion is always perfect in MS

The purpose of the database search is to find exact or nearly exact matches